Chilean rose tarantulas are nocturnal. They prefer dry dessert areas and will burrow in the wild but have not been known to burrow in captivity. They have a diverse diet including grasshoppers, crickets, moths, beetles, cockroaches, mealworms, small lizards and mammals.
Males will approach the female’s burrow with caution, tapping and vibrating his legs to attract her out. The female can lay an egg sack that holds up to 500 spiderlings.
In defense, Chilean rose tarantulas will raise its front legs, and present its fangs in preparation to defend itself. The venom contains multiple toxins, which may help it immobilize and digest prey, as well as deter predators. A specific peptide found in this venom, termed GsMTx4 (Grammostola rosea Mechanotoxin 4) has been shown to inhibit mechanosensitive ion channels in living cells.
It has small, spine-like urticating hairs on its abdomen that it kicks off or releases when threatened as a defense.
They have a life span of approximately 20 years. Their conservation status is of Least Concern on the IUCN Red List.